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Posted: **Thu Dec 02, 2021 5:42 am**

: 1 Define The Followings A Chromophore B Red Bathochromic Shift 2 What Are The Possible Electronic Transitions T 1 (149.27 KiB) Viewed 91 times

: 1 Define The Followings A Chromophore B Red Bathochromic Shift 2 What Are The Possible Electronic Transitions T 2 (104.22 KiB) Viewed 91 times

1- Define the followings: a) Chromophore: b) Red (Bathochromic) shift: 2) What are the possible electronic transitions that could be occurred in the following cases? (you can write them down as words (sigma, pi,...) if you do not find symbols) a) Saturated compound has atoms with lone pairs: b) HCN: c) Methane: 3) What are the HOMO and LUMO in each case in Question 2? HOMO LUMO b)

4) Apply Beer - Lambert law, to calculate the conc. For Fluorescein using the below data and the suitable (8) value from your manual. For Fluorescein: A= 0.298 at 1 = 495 nm For Fluorescein: A= 0.276 at 1 = 500 nm 0.6- 0.5- 0.4 88704 SITO 0.3- Abs 0.2- 0.1- 0.0- 400 500 Wavelength (nm) 600