4. (2 points) Briefly explain why each of the following experimental results suggest that the proteins being analyzed ar
Posted: Thu Dec 02, 2021 5:41 am
4. (2 points) Briefly explain why each of the following experimental results suggest that the proteins being analyzed are oligomeric. (a) The protein catalase has a molecular weight of 232,000 in SDS-PAGE gels run without 2- mercaptoethanol (2ME). When catalase is pre-treated with 2ME, its molecular weight appears to be around 80,000 in SDS-PAGE gels. (b) When the amino acid sequence of the protein abrin was determined, two different N-terminal amino acids (serine and glutamic acid) were identified. 5. (2 points) Briefly explain why heating a protein destroys its secondary and tertiary structures but not its primary structure.