devR hspx Fold induction wrt controls 6000- 5000- 4000- 3000- 1000- 800- 600- 400- 200- 0 LIX48 LIX68 (H37Ra::pJFR19-dev
Posted: Tue May 31, 2022 11:10 pm
- Devr Hspx Fold Induction Wrt Controls 6000 5000 4000 3000 1000 800 600 400 200 0 Lix48 Lix68 H37ra Pjfr19 Dev 1 (69.38 KiB) Viewed 38 times
controlled by DevR and PhoP. The strain LIX48 overexpresses (makes
a lot of) DevR but not PhoP, while the strain LIX68 overexpresses
both DevR and PhoP. The fold induction is relative to a strain
which does not overexpress either gene.
QUESTION: Based on the Figure above answer
the two following questions:
(i) what happens to expression at the promoters devR
and hspX when DevR is overexpressed (left set of
bars)?
(ii) What happens to expression at those promoters when both
DevR and PhoP are overexpressed (right set of bars)?
devR hspx Fold induction wrt controls 6000- 5000- 4000- 3000- 1000- 800- 600- 400- 200- 0 LIX48 LIX68 (H37Ra::pJFR19-devR) (H37Ra::pJFR19-devR + pMV261-phoPRV) Figure 3. qRT-PCR analysis of M. tuberculosis H37Ra devR overex- pression strain complemented with phoPRV gene (LIX68). -Fold induc- tion of devR and hspX genes in LIX68 and its parent strain (LIX48) with respect to EV control strains (baseline expression set to 1.0) is shown. Results are presented as mean ± S.D. (error bars) of three independent experiments.