Answer Happy

A eukaryote pre-mRNA shown is alternatively spliced, resulting in multiple mature mRNA isoforms (Figure 1A below). In order to detect these, you perform a Northern blot on the spliced mature mRNA using Probes 1, 2 and 3 that bind in the indicated positions. For probes 1 and 3, you observe the banding patterns shown in Figure 1B below. (a) Using the banding patterns for probe 1 and 3, list all possible splice variants. (b) Based on your previous answer, draw the banding pattern you would expect from a Northern blot performed with Probe 2. Figure 1. Exon 1,500 bases A Exon 2, 100 bases Exon 3, 200 bases Exon 4, 800 bases Probe 1 Probe 2 Probe 3 B Probe 1 Probe 2 Probe 3 1600- 1400- 1200- 1000- 800 |