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7. Explain why the trp operon is described as a negative repressible operon. Discuss how the regulatory protein enables this type of regulation of the trp operon. 8. Indicate whether each of the following events will occurs under conditions of low tryptophan or of high tryptophan, motivate your answer. a. b. Ribosome does not stall at trp codons Region 2 of the leader pairs with region 3 Ribosome covers part of region 2 of leader C. d. Transcription is not terminated prematurely and the structural genes are transcribed. 9. Consider the following three mutations that in the 5' UTR region of the trp operon of E. coli. Describe the most likely effect each of these mutations will have on the transcription of the trp structural genes. a. A mutation where the AUG start codon for the 5' UTR peptide has been deleted. b. A mutation that changes the tryptophan codons in region 1 of the mRNA 5' UTR into codons for cysteine C. A deletion of region 3 of the mRNA 5' UTR 10. Refer to Fig 16.17. Which specific sequences in the 5' UTR of the ompF mRNA do you think micF RNA binds? Motivate your answer. (See Chapter 15 for information about ribosome binding and initiation of translation.)