Isobutanol (C4H10O; MW=74.12) is an interesting biofuel due to its attractive properties such as its high energy content
Posted: Tue May 24, 2022 11:24 am
Isobutanol (C4H10O; MW=74.12) is an interesting biofuel due to
its attractive properties such as its high energy content and
compatibility with gasoline engines. I would like to you think
about producing this fuel using engineered E. coli cells
(CH1.75O0.5N0.16). Your carbon and nitrogen sources will be glucose
(C6H12O6; MW=180) and ammonia (NH3), respectively. Experiments in
lab-scale bioreactors showed that the following cell and product
yields can be achieved: YX/S = 0.15 g cell/g glucose, YP/S = 0.14 g
isobutanol/g glucose.
a. Assuming that cell growth and isobutanol production
occurred simultaneously, write a balanced stoichiometric reaction
for this biological process. (92% of the E. coli dry cell weight is
composed of C, H, O, and N. Their atomic masses are 12, 1, 16 and
14, respectively.)
b. What is the product yield on cells (YP/X; g isobutanol/g
cell)?
its attractive properties such as its high energy content and
compatibility with gasoline engines. I would like to you think
about producing this fuel using engineered E. coli cells
(CH1.75O0.5N0.16). Your carbon and nitrogen sources will be glucose
(C6H12O6; MW=180) and ammonia (NH3), respectively. Experiments in
lab-scale bioreactors showed that the following cell and product
yields can be achieved: YX/S = 0.15 g cell/g glucose, YP/S = 0.14 g
isobutanol/g glucose.
a. Assuming that cell growth and isobutanol production
occurred simultaneously, write a balanced stoichiometric reaction
for this biological process. (92% of the E. coli dry cell weight is
composed of C, H, O, and N. Their atomic masses are 12, 1, 16 and
14, respectively.)
b. What is the product yield on cells (YP/X; g isobutanol/g
cell)?