Answer Happy

5. Read the entire description below before attempting to answer the questions that follow. E G G Virion RNA TILV RNA NNV RNA E-11 RNA Mock panel D. no cytopathic effect (experimental condition) panel E: cytopathic effect observed (control) panel F. no cytopathic effect (control) panel G: no cytopathic effect (control) General background: researchers attempted to identify and characterize a novel virus suspected of causing mass die-offs of certain fish species. The study authors isolated the virus and called it tilapia lake virus (TILV). Initial characterization of the genome showed that it consisted of single-stranded RNA Information to help understand the experimental desien used in the figure above panel D: purified RNA fie.. no viral proteins present) from TILV Oos was introduced into 8-11 cells (a cell line derived from a fish) to find out if cytopathic effect occurred panel E: purified RNA (ie, no viral proteins present) from nervous necrosis virus (NNV) was introduced into E-11 cells as in panel D this condition served as a positive control panel F: purified RNA from an aliquot of E-11 cells was prepared and then introduced into another sample of E-11 cells: this condition served as a negative control d.o., no Cytopathic effect was expected) panel G: untreated and uninfected ("mock" cells: this condition served as a negative control (.e., no cytopathic effect was expected) (a) Based on the data, does Till contain a positive or negative sense genome? Explain your reasoning, 13 pts. 1 (b) Design an experiment to figure out if the genome of TILV is segmented or non-segmented. Explain your rationale and how the experiment would address the question. [3 pts.]