Answer Happy

You are analyzing the region of DNA shown below to determine how many AATG repeats are present. To do so, you must amplify the entire region of AATG repeats. Design primers of 16 bases each so they anneal outside the region of interest. More than one primer pair is possible, but just give one. Hint: highlight the repeats and then count back from there 5'-ACTGGCACAGAACAGGCACTTAGGAATGAATGAATGAATGAATGAATGAATGACCTGTGTGGTTCCCAGTTCCTCC-3' 3' -TGACCGTGTCTTGTCCGTGAATCCTTACTTACTTACTTACTTACTTACTTACTGGACACACCAAGGGTCAAGGAGG-5' a. Primer 1: Primer 2: b. Highlight the repeats in yellow. c. How many repeats of the AATG sequence are there? 5. PCR Product size You use the primers below in a PCR with the DNA in question 3 above. 5'-CTGGCACAGAACAGGCACTT-3' and 3'-ACACACCAAGGGTCAAGGAG-5' a. How long (in base pairs) will the PCR product be? b. How long would the PCR product be if there were 5 repeats? What about if there were 10 repeats?