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Question 2 1 pts After cloning an insert into a plasmid, determining its orientation is best accomplished with ... Two restriction endonucleases that cut in the insert. O A single restriction endonuclease that cuts twice to release the insert. Two restriction endonuclease, one that cuts once within the insert and the other that cuts once in the plasmid backbone. O A single endonuclease that cuts twice in the plasmid backbone. Question 3 1 pts Which of the following best describes the action of ligase? O It catalyzes the transformation of bacteria to an antibiotic-resistant state. O It makes a double-strand cut in DNA at a specific sequence. It catalyzes the annealing of complimentary molecules of single-stranded DNA. O It catalyzes the formation of phosphodiester bonds.