Answer Happy

The diagram below shows two DNA molecules, a plasmid for cloning and an insert - the gene encoding green fluorescent protein (GFP). Your task is to clone GFP into the EcoRI site of the plasmid and analyze the resulting putative clone. Consider scenarios A and B below and answer the questions. A. The insert is oriented in the same direction as the AmpR gene (5'-3'). B. The insert is oriented in the opposite direction as the AmpR gene (3-5'). EcoRI 800 bp Xhol EcoRI 200 bp 1 1 Green Fluorescent Protein 1 EcoRI 121 bp 702 bp 177 bp 450 bp Amp Pvull Plasmid: 3600 bp ---- 1. Under scenario A, what is the result of cutting the recombinant plasmid with EcoRI? [Select ] 2. Under scenario B, what is the result of cutting the recombinant plasmid with Xhol? [Select ] 3. Under scenario A, what is the result of cutting the recombinant plasmid with Xhol + Pvull? [ Select ] 4. Under scenario B, what is the result of cutting the recombinant plasmid with Xhol + Pvull? Select ] 5. What is the size of the GFP protein, assuming that the coding sequence (including stop codon) is 702 bp? [Select]