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13. When molecular biologists carry out a PCR, they have a range of different DNA polymerase enzymes on the market that
Posted: Mon May 23, 2022 8:30 am
by answerhappygod
- 13 When Molecular Biologists Carry Out A Pcr They Have A Range Of Different Dna Polymerase Enzymes On The Market That 1 (95.76 KiB) Viewed 14 times
13. When molecular biologists carry out a PCR, they have a range of different DNA polymerase enzymes on the market that they can purchase, some of them complete proteins taken from different species, and some modified proteins. A molecular biologist plans to buy a Taq DNA polymerase to PCR a DNA fragment for cloning into an expression vector. a. Which two polymerases are used during DNA replication in the cell? (1 mark) b. List the different enzymatic properties of these DNA polymerases and explain the specific situations they would be used during DNA replication. (3 marks) c. Explain which enzymatic properties a molecular biologist might want in a Taq polymerase used for the experiment described above. (1 mark)