Answer Happy

Now, based on the 10-bp DNA molecule you created, use the agarose gel illustration below to draw the results of electrophoresis of: 1. your original DNA molecule (in the well where no restriction enzyme was added, "-") 2. your digested DNA (in the well where restriction enzyme was added, "+") 3. Be sure to add an appropriate DNA molecular weight ladder series in well "M" (including labeling the number of bp in each band you invent) M +

8 (10 points) Create and write out both strands of a 10-bp piece of DNA that contains one of the restriction sites below. The /(slash) character in the restriction site is used to indicate where the appropriate restriction enzyme will cut both DNA strands at each restriction site. Label your double-stranded DNA molecule with appropriate polarity notation. Clearly show the location and name of the restriction site in your double-stranded DNA molecule. Alul AG/CT Bsti CG/CG Dral TTT/AAA EcoRV GAT/ATC Hpal GTTAAC Smal CCC/GGG