Place the steps of our transformation experiment in order from start to finish. Transfer your solutions to the agar plat
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Place the steps of our transformation experiment in order from start to finish. Transfer your solutions to the agar plat
solutions to the agar plate to observe the growth of the bacteria. Heat shock the solution to create pores in the bacterial wall/membrane. Combine the E. coli, pGlo plasmid and calcium chloride solution. Place the solutions on ice to keep the plasmid DNA inside the bacteria Allow for the DNA and bacteria to come together with the calcium ions acting as a scaffold.
Place the steps of our transformation experiment in order from start to finish. Transfer your