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25. You are a researcher who wishes to isolate and remove a gene from a human chromosome and to incorporate it into a plasmid vector. Is it important to use the same restriction enzyme to remove the gene from the chromosome and to open up the plasmid ring? Explain your answer. [2 marks]

19. A drug becomes attached to the repressor protein and prevents the protein from binding to the operator region. Describe how this would affect the transcription of the lac operon. [1 mark]

23. In an experiment, a prokaryote is exposed to high intensity UV light, which causes a mutation in a gene. The mutated gene synthesizes a much shorter polypeptide chain then the normal gene. What type of mutation is this? Explain [2 marks]