Answer Happy

A geneticist want to isolate the gene from a tick that encodes
the anti-coagulant protein found in its saliva which prevents human
blood clotting, with the purpose of transferring this gene to
bacteria and producing the anti-coagulant for medicinal
purposes.
a) Should the geneticist construct a tick genomic or cDNA
library? Motivate fully.
b) Which tick tissue will be most suitable as source of the
nucleic acids used for library construction? Explain.
c) A partial amino acid sequence from the tick anti-coagulant
protein is: …..
Tyr-Met-Ser-Arg-Phe-Val-Tyr-Lys-His-Cys-Met-Leu-Ile-Arg-Thr-Pro …..
You wish to make a set of DNA probes to screen your tick library
for the clones containing the sequence that encodes this protein.
Your probes should be 15 nucleotides in length.
3 Which amino acids in the protein should be used to construct
the probes so that the least degeneracy results (consult the
genetic code)? How many different probes must be synthesized to be
certain that you will find the correct sequence that specifies the
protein? Give the nucleotide sequence of any one of these
probes.