URGENT
Posted: Mon May 23, 2022 7:18 am
URGENT
Suppose that you want to sequence the following two DNA fragments: (1) (2) 5'-GCGCAATTCTTC-primer site-3' 3'-primer site -AACCGGTACTAC-5' In each case you first use PCR to amplify the fragment, so that there is sufficient DNA for sequencing. You carry out Sanger dideoxy sequencing with a primer that anneals to the primer site, and then separate the products of the four sequencing reactions by gel electrophoresis. Draw the bands that should appear on the gel from the sequencing reactions for fragments (1) and (2) respectively. (1) Reaction containing ddATP ddCTP ddGTP ddTTP (2) Reaction containing ddATP ddCTP ddGTP ddTTP
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