Question 2 You can use a graph paper or excel to answers parts of this question. The enzyme cytochrome c reductase reduc
Posted: Mon May 23, 2022 6:43 am
- Question 2 You Can Use A Graph Paper Or Excel To Answers Parts Of This Question The Enzyme Cytochrome C Reductase Reduc 1 (47.12 KiB) Viewed 22 times
Question 2 You can use a graph paper or excel to answers parts of this question. The enzyme cytochrome c reductase reduces oxidized cytochrome c in the presence of decylubiquinol. The reaction can be followed spectrophotometically at 550 nm. The kinetics of this enzyme was investigated at fixed amount of enzyme at saturated decylubiquinol in the presence and absence of Hg (4 µM). Table 1 gives the data generated by this experiment. Answer the questions below: Table 1 [cyt.c] (mm) 0.083 - Hg Vo (AAbs/min) + Hg (4 µM) 1.32 2.27 0.1 2.56 1.5 0.125 2.86 1.74 0.167 3.38 2.08 0.25 4.08 2.56 0.5 5.0 3.33 (a) To which EC class does this enzyme reaction belong to? (1) (b) Investigate the data to determine if mercury (Hg) is an inhibitor or not. If it is indeed and inhibitor, classify it as an inhibitor by using all possible kinetic data to fully support your answer - including inhibition constant/s (19)