Answer Happy

Short Answer Providing details and explaining your reasoning may help you get partial credit. Show all work on calculations. 1. Consider the following mechanism: CHOPOCHOPO H HO OH OH H Box 1 CH4₂0PO3 HCH,OPO kháo HA H₂0 HO'CH :B- Bi Han H'COH ÁCH,OPOF H Box 3 CH,OPOST CHO CH₂OH ما CH,OPOF H HOCH ¡A- 18- HON CH,OPOT HCOH CHOPOS Box 2 HOOH CHOPO HOCH HC-H HCOH CHO PO <-8H CHORO H -- Lys-N: A- H-A- H HOCH 18- H-B- a. (2 pts) What kind of reaction is occurring during this step of glycolysis? A- A- H-8-

b. (2 pts) What metabolite is in Box 1? c. (2 pts) What metabolite is in Box 2? d. (2 pts) What metabolite is in Box 3? e. (2 pts) What enzyme catalyzes this reaction? f. (2 pts) Circle and label one place on the mechanism where covalent catalysis is occurring. g. (4 pts) Suggest an amino acid that could fill the role of residue "A" in the mechanism above and draw its structure at pH 7.4. h. (4 pts) Suggest an amino acid that could fill the role of residue "B" in the mechanism above and draw its structure at pH 7.4. i. (2 pts) Under standard conditions this reaction is unfavorable (AG" = 23.8 kJ/mol). What conditions in the cell allow for the actual free energy change to be lower (AG= 0), making the reaction readily reversible? Explain your answer.