- Determine the primary structure of a hexapeptide from the following: Acid-catalyzed hydrolysis gives 2Arg, Leu, Lys, M
Posted: Sun May 22, 2022 10:33 pm
- Determine the primary structure of a hexapeptide from the following: Acid-catalyzed hydrolysis gives 2Arg, Leu, Lys, Met, Ser. Use of carboxypeptidase A releases Ser. Edman's reagent releases Leu. Trypsin-catalyzed hydrolysis forms: 1. Arg. 2. Ser, 3. Arg, Met, 4. Leu, Lys; Cyanogen bromide treatment forms: 1. Arg, Ser, and 2. Arg, Leu, Lys, Met. Also, show the mechanisms for both the Edman's reagent and the cyanogen bromide.