Answer Happy

1. Consider the following mechanism: CHOPO CHOPO HHO H OH OH H Box 1 CH,OP - HICHOPOS H-A- 1 GHzopo? * L- NO HjO H Low PA S HOICH LO-HIA нносн -B2 H-O-H HOCH HC.COM -B: H-O-H нсон CH,OPo". - нсон -BH нсон CH,Ovo- CH,OPO- Box 2 Box 3 CH,OPO co CH, OH нсон CH,OPO" CHOPOS H Lys-N H HO 1 CH,OPO" L-- HOCH IA H-A- LyN IA *** -- H H- -- -BH

g. (4 pts) Suggest an amino acid that could fill the role of residue "A" in the mechanism above and draw its structure at pH 7.4. h. (4 pts) Suggest an amino acid that could fill the role of residue “B” in the mechanism above and draw its structure at pH 7.4. i. (2 pts) Under standard conditions this reaction is unfavorable (DG¢ = 23.8 kJ/mol). What conditions in the cell allow for the actual free energy change to be lower (DG » ), making the reaction readily reversible? Explain your answer.