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22. (3 points) Which type of membrane protein might be dissociated from the membrane by changing the pH or the ionic strength? (choose one answer): a. Integral membrane protein b. Peripheral membrane protein C. Lipid anchored membrane protein d. All of the above - 23. (3 pts) The oxygen binding affinity of hemoglobin decreases at lower pH because (choose one answer) a. Protonated side chains form ionic bonds which stabilize the T-state. b. Unprotonated side chains form ionic bonds which stabilize the T-state. C. Protonated side chains form ionic bonds which stabilize the R-state. d. Unprotonated side chains form ionic bonds which stabilize the R-state. Extra Credit - 3 extra points Which redox reaction is possible? (choose one answer) o CH3 -CH-CH3 + NAD → CH-C-CH3 + NADH+H* 0 IL b. CH3 -C-OH+ NAD + 3H → CH3 -C-H + NADH + H2O o o CH-2-OH + HPO 2 + NAD → CH3-C-O-P-O + NADH + H2O OH a. - 11 c. Extra Credit - 4 extra points The kinetics of an enzyme were measured in the presence and absence of inhibitor. For this enzyme, Km = 33 mM and Vmax = 55 mmol/sec. In the presence of inhibitor Y (10 mm), the measured km = 6 mM and the measured Vmax = 10 mmol/sec. Calculate Ki for inhibitor Y S No Inhibitor With Inhibitor Y (mm) Vo (mm/s) Vo (mm/s) 2 3.14 2.50