Answer Happy

Calculate the actual, physiological AG for the reaction below at 37°C with phosphocreatine at 4.7mM, creatine at 1.0mM, ADP at 0.73mM and ATP at 2.6mM. Show your work for full marks. Note: Use the table of “Free energies of Hydrolysis of some high-energy compounds” on the last page of the assignment to answer the question. Phosphocreatine + ADP → creatine +ATP

TABLE 3.3 Free Energies of Hydrolysis of Some High-Energy Compounds* 4G: Compound and Hydrolysis Reaction (kl/mol) Structure Phosphoenolpyruvate — pyruvate + P -62.2 Figure 3.12 1,3-Bisphosphoglycerate —— 3-phosphoglycerate + P. -49.6 Figure 3.10 Creatine phosphate — creatine + P, -43.3 Figure 13.21 Acetyl phosphate acetate + P -43.3 Figure 3.10 Adenosine-5'-triphosphate — ADP + P. -35.7 Figure 3.9 Adenosine-5'-triphosphate - ADP + P - 30.5 Figure 3.9 (with excess Mg?) Adenosine-5'-diphosphate AMP+P -35.7 Figure 3.9 Pyrophosphate —P; + P (in 5 mM Mg2+) -33.6 Figure 3.8 Adenosine-5-triphosphate AMP+ PP (excess Mg2+) -32.3 Figure 10.14 Uridine diphosphoglucose — UDP + glucose -31.9 Figure 22.14 Acetyl-coenzyme A-acetate + COA -31.5 S-adenosylmethionine —— methionine + adenosine -25.6 Figure 25.28 Glucose-1-phosphate glucose + P -21.0 Figure 7.13 Sn-Glycerol-3-phosphate glycerol + P -9.2 Figure 8.5 Adenosine-5'-monophosphate- adenosine + P -9.2 Figure 10.11 TABLE 17.2 Steady-State Concentrations of Glycolytic Metabolites in Erythrocytes Metabolite mM Glucose 5.0 Glucose-6-phosphate 0.083 Fructose-6-phosphate 0.014 Fructose-1,6-bisphosphate 0.031 Dihydroxyacetone phosphate 0.14 Glyceraldehyde-3-phosphate 0.019 1,3-Bisphosphoglycerate 0.001 2,3-Bisphosphoglycerate 4.0 3-Phosphoglycerate 0.12 2-Phosphoglycerate 0.030 Phosphoenolpyruvate 0.023 Pyruvate 0.051 Lactate 2.9 ATP 1.85 ADP 0.14 P. 1.0 page 616