1. Glucose in the urine, glycosuria, can be an indicator of Diabetes. Which experiment (#1, #2 or #3) would you use to t
Posted: Sun May 22, 2022 4:11 pm
1. Glucose in the urine, glycosuria, can be an indicator of Diabetes. Which experiment (#1, #2 or #3) would you use to test suspect urine for the presence of sugars?
Experimental Results:
#1 Sugar Test with BENEDICT’S Solution: The following carbohydrates (Fructose, Sucrose, Glucose, Starch, Xylose, and Maltose) were added to test tubes in the presence of Benedict’s solution. The results are heating are as follows (below):
F = fructose Su = sucrose Gl = glucose St = Starch X = xylose M = maltose
#2 Starch Test following Acid catalyzed Hydrolysis and Oxidation with Benedicts of STARCH. An acidic solution of Starch, was heated for 1-2 hours. Each 15 minutes, a sample was removed, added to a chem plate and the IKI solution added. The solution was heated until the starch test became negative. At this point, the Sugar Test (Benedicts was repeated). Results are below:
These results are shown on the left-side chemplate. The results of the initial Benedict’s test with starch (left test tube) and after hydrolysis (right test tube) are shown above on the right.
#3 Acid and Base Hydrolysis. Test tubes (left basic NaOH), (right acid H2SO4) that contained sucrose (a disaccharide) were heated to hydrolyze the sucrose into monosaccharides. The benedicts test was repeated to determine if hydrolysis occurred as shown by a positive Benedicts test detecting the oxidation of the newly formed monosaccharides.
Test Tube Left: basic conditions,
Test Tube on the Right: Acidic Conditions
Experimental Results:
#1 Sugar Test with BENEDICT’S Solution: The following carbohydrates (Fructose, Sucrose, Glucose, Starch, Xylose, and Maltose) were added to test tubes in the presence of Benedict’s solution. The results are heating are as follows (below):
F = fructose Su = sucrose Gl = glucose St = Starch X = xylose M = maltose
#2 Starch Test following Acid catalyzed Hydrolysis and Oxidation with Benedicts of STARCH. An acidic solution of Starch, was heated for 1-2 hours. Each 15 minutes, a sample was removed, added to a chem plate and the IKI solution added. The solution was heated until the starch test became negative. At this point, the Sugar Test (Benedicts was repeated). Results are below:
These results are shown on the left-side chemplate. The results of the initial Benedict’s test with starch (left test tube) and after hydrolysis (right test tube) are shown above on the right.
#3 Acid and Base Hydrolysis. Test tubes (left basic NaOH), (right acid H2SO4) that contained sucrose (a disaccharide) were heated to hydrolyze the sucrose into monosaccharides. The benedicts test was repeated to determine if hydrolysis occurred as shown by a positive Benedicts test detecting the oxidation of the newly formed monosaccharides.
Test Tube Left: basic conditions,
Test Tube on the Right: Acidic Conditions