The thermophilic archaebacterium Thermoproteus tenax expresses two GAPDH enzymes: one that carries out the reaction show
Posted: Wed Nov 24, 2021 10:42 am
In an experiment, the activity of the second T. tenax GAPDH enzyme was measured in the presence of various metabolites. The results are shown in the figure below. Estimate the KM values for the enzyme in the presence and absence of effectors. 25 20 Velocity (units .mg protein) 5 1 J 5 10 15 20 [NAD*] (MM) NADP+ + Control AMP * Glucose-1-P KM (Control) is approximately mM. KM (NADP+) is approximately mM. 3.0 0.5 5.0 1.5 KM (AMP) is approximately M. KM (G1P) is approximately 4 mm.
Using the results in the graph shown below, classify the effectors as activators or inhibitors of the enzyme. 25 20 15 Velocity (units .mg protein) 10 5 1 thie 10 5 15 20 [NAD*] (MM) Control + AMP * Glucose-1-P NADP+ NADP+ is ar AMP is an activator inhibitor G1P is an