b) Substances A and B have retention times of 16.50 and 18.20 minutes, respectively, on a 30 cm column. An unretained sp
Posted: Wed Nov 24, 2021 10:33 am
b) Substances A and B have retention times of 16.50 and 18.20 minutes, respectively, on a 30 cm column. An unretained species passes through the column in 1.30 minutes. The peak widths (at base) for A and B are 1.21 and 1.32 minutes, respectively. (6 marks) Calculate i) the time spent by A and B on the column ii) column resolution iii) average number of plates in the column iv) the plate height v) the length of the column required to achieve a resolution of 1.50 2[(tr):-(tx)] W,+W N = 16 46 ) (Rs),- _N (Rs), = VN, L H = N R W