Answer Happy

H. (15 points) The set of data shown below is a cytotoxicity assay. The cytotoxicity assay is NOT an MTT colormetric assay, but is a 96 well plate assay nonetheless, with all the experimental design identical to a 96 well MTT plate assay. The cell line is AC (control/ non-pretreated) while AC are cells pretreated with hormone(X) BEFORE it has been exposed to the 6 (0.0125 to 12. 5 uM) different antibiotic treatments shown below (rimsulfuron, sprioxamine, triadimenol, paclobutrazol, bifenazate, picardian). The IC 50 is given where applicable as AC50 =0.463, 11.5, 23.1 or where it cannot be determined it is AC = ND a. 46 The cytotoxicity assay plate is shown in A on the left. Discuss the "rights" and any "wrongs of the plate design (controls, vehicles, wells, rows, set ups etc) b. Discuss the pretreatment of the cells with hormone X, on the cytotoxicity of the antibiotic treatments shown in B. Provide a BRIEF overall, GENERAL, conclusion/summary for the results in this experiment. C. A А 0.0125 UM Vehicle Media 10.125 UM 1.25 UM 12.5 UM Media 17 Rimsulfuron 18 19 Spiroxamine 20 Triadimenol 21 Paclobutrazol 2200 Bifenazate 23 Picaridin 24 DRAQ5/Sapphire 700 G-MYH6/MYH7 goat a-mouse (CARDIOMYOCYTE DIFFERENTIATION) (CYTOTOXICITY) B Rimsulfuron Spiroxamine Triadimenol 120 120- 100- 140- 120- 100 of Control RO of Control 800- 30- 60- 40- 20- 0 0.01 40- 20- 0+ 0.01 0.1 110 Treatment Concentration (M) TOO 100 0.01 100 10 Treatment Concentration (M) 10 Treatment Concentration (M) AC50=ND AC50=ND Paclobutrazol AC50=ND AC50=0.463 Bifenazate AC50=ND AC50=11.5 Picaridin 120- 120 1004 of Control BO 60 40- 20 0-+ 0.01 % of Control 100- B0- 80- 40- 20- of Control 10 100 0.01 0.01 160 0.1 Treatment Concentration (M) () 01 Treatment Concentration (M) 100 Treatment Concentration (M) AC50-ND AC50=ND AC50=ND AC50=23.1 AC50=ND AC50=ND