QUESTION 2 [29] 2.1. The turnover number is denoted by the term kcat. What is the significance of determining this value
Posted: Wed May 18, 2022 1:52 pm
QUESTION 2 [29]
2.1. The turnover number is denoted by the term kcat. What is
the significance of determining this value? (5)
2.2. As a researcher you wish to know how well your newly
discovered enzyme works compared to other known enzymes. Which
catalytic parameter(s) will you determine and why? (6)
2.3. What does the term “salting in” mean? How does it work in
the context of enzymes? (4)
2.4. Explain why the thermodynamic parameter ΔS cannot be used
to predict the direction in which a reaction will proceed. (4)
2.5. All enzyme kinetic parameters are determined in vitro. What
are the implication of studying enzyme kinetics in vivo? (5)
2.6. What determines the ultimate speed limit of an
enzyme-catalysed reaction, i.e. why does it reach a limit and not
go on to accelerate a reaction indefinitely? (5)
2.1. The turnover number is denoted by the term kcat. What is
the significance of determining this value? (5)
2.2. As a researcher you wish to know how well your newly
discovered enzyme works compared to other known enzymes. Which
catalytic parameter(s) will you determine and why? (6)
2.3. What does the term “salting in” mean? How does it work in
the context of enzymes? (4)
2.4. Explain why the thermodynamic parameter ΔS cannot be used
to predict the direction in which a reaction will proceed. (4)
2.5. All enzyme kinetic parameters are determined in vitro. What
are the implication of studying enzyme kinetics in vivo? (5)
2.6. What determines the ultimate speed limit of an
enzyme-catalysed reaction, i.e. why does it reach a limit and not
go on to accelerate a reaction indefinitely? (5)