Answer Happy

pH = 7 pH = 7 pH = 7 pH = 5 pH = 9 Environ mental Conditi ons: Temper ature (°C) 15 [S] =90 mM V (mM/min [S] = 90 mM V (mm/mi [S] = 60 mM V (mm/mi n) 0.00089 0.00137 0.00200 0.00245 [S]=120 mM V (mM/mi n 0.00095 0.00161 0.00230 0.00288 0.00311 [S] =90 mM V (mm/mi n 0.00060 0.00097 0.00138 0.00163 30 0.00124 0.00205 0.00300 0.00357 0.00395 0.00375 0.00346 0.00096 0.00158 0.00222 0.00278 32 35 37 0.00275 0.00300 0.00180 39 0.00261 0.00300 0.00293 41 0.00256 0.00283 0.00267 43 0.00306 0.00231 0.00245 0.00209 45 0.00272 0.00176 0.00147 0.00177 0.00162 0.00146 0.00123 0.00100 0.00078 0.00055 48 0.00167 0.00241 0.00212 0.00171 0.00131 0.00094 0.00224 0.00165 50 0.00120 0.00138 55 0.00121 0.00090 0.00104 TA
a 12. Using the data in Table 1, plot V vs. temperature at pH 7 and a substrate concentration of 90 mM (graph not to be included in your assignment), and then answer the following questions. (6 marks) a. What is the optimal temperature for amylase activity? b. Would amylase isolated from any two organisms (e.g., fish amylase vs. amylase from the small intestine of humans) show the same temperature optimum? Explain your answer.
14. If you were to carry out these temperature experiments at a higher or lower [S] and pH 7, do you think that the temperature optimum for amylase would change? Formulate a hypothesis that addresses this issue. Using the data from Table 1, test your hypothesis (graph not to be included in your assignment). Briefly explain your answer. (6 marks) 15. If you were to carry out these temperature experiments at a higher or lower pH and 90 mM (S], do you think that the temperature optimum would change? Formulate a hypothesis that addresses this issue. Test your hypothesis using the data in Table 1 (graph not to be included in your assignment). Briefly explain your answer. (6 marks)