You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) 0 1 2 3 4 5 Absorbancy 595nm
Posted: Wed May 18, 2022 10:06 am
- You Obtained The Following Raw Data When Setting Up A Bradford Standard Curve Bsa Mg Ml 0 1 2 3 4 5 Absorbancy 595nm 1 (17.88 KiB) Viewed 59 times
You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) 0 1 2 3 4 5 Absorbancy 595nm 0.225 0.310 0.420 0.510 0.610 0.720 0.810 0.915 0.950 0.980 0.990 6 7 8 9 10 After blanking against a bradford-dH 20 sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.665 was obtained Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg/ml (up to 3 significant figures).