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Quemon Completion Status QUESTION 20 por Both Buret and Bradford methods was used to determine unknown concentrations of both BSA and Big The methods followed is described in detail in the Background section, The following results was obtained: Table 1: Absorbance at 595mm obtained for different concentrations of protein standards using the Bradford method [Protein (mg/ml) 0.00 0.125 0.25 0.50 0.75 1.00 1.50 2.00 Unknown Abs. BSA 0 0.130 0.350 0.620 0.870 1.120 1.400 1.60 1.050 Abs, Big 0 0.080 0.130 0.360 0.520 0.650 0.850 1.080 13 16 15 14 13 A SAA 1.1 10 Abortancy ON 07 OB
Question Completion Status Table 1: Absorbance at 505nm obtained for different concentrations of protein standards using the Bradford method [Protein) 0.00 0.125 (mg/ml) 0.25 0.75 1.00 1.50 2.00 Unknown 0.50 Abs. BSA 0 0.130 0.350 0.620 0.870 1.120 1.400 1.80 : 1.050 Abs, Big 0 0.080 0.130 0.360 0.520 0.650 0.850 1.080 18 1.7 16 ABSA --A. BE 14 Abstancy 55 06 04 0.1 0.00 0.25 0.50 150 1.75 2.00 0.75 100 125 Protein concentration ( mm)
Question completion Status Table 2: Absorbance at 540nm obtained for different concentrations of protein standards using the Buret method [Protein! 0.00 0.125 0.25 (mg/ml) 0.50 0.75 1.00 1.50 2.00 Unknown Abs. BSA 0 0.020 0.045 0.075 0.111 0.1280.172 0218 0.26 Abs. Big O 0.024 0.052 0.092 0.136 0.162 0.223 0.264 0.26 0.25 024 A A A 0.22 0.20 U18 0:16 bsorbancy 1540nm 014 0:12 0.10 006 004 0.00 000 0.25 050 0.75 200 8 125 150 175 200
bsorba 0.12 0.10 0.08 0.06 0.04 0.02 0.00 0.00 0.25 0.50 0.75 1.00 150 1.75 2.00 1.25 Protein concentration (me/ml) Which standard curve would you use to determine the concentration of the Unknown protein sample? BSA standard curve using the Bradford method Big standard curve using the Bluret method O Big standard curve using the Bradford method OBSA standard curve using the Bluret method