Answer Happy

1.Delete the exons using a cloning vector, a 56-base pair origin of replication, and
of replication of 56 base pairs, and antibiotic resistance of 30 base pairs.
30 base pairs.
2. Locate a mutation and remove it in another cloning vector using a fluorochrome
using a 48 base pair fluorochrome instead of antibiotic.
base pairs fluorochrome instead of antibiotic.
3. Indicate the type of cloning vector used and the reason for this choice.
reason for this choice.
4. indicate at what temperature you would incubate this vector and how long you expect it to take to
how long we expect the growth to occur.
Give reasons for your answer.
Find the appropriate restriction enzyme from the following
Endonuclease BamHI MATTC Sochy Eco RI Hind III DNA sequence Cleavage products GGATCC GATCC Sticky CCTAG CCTAG G ends GAATTC CTTAG CTTAA G ends AAQCTT A AGCTT Sticky TTCGAN TTCGA AL ends GCC GG ce Blunt CCGG се GG ends CTGCA CTGCA G Sticky GACGTC ACGTC ends CCCGGG CCC GGG Blunt GGGCCC GGG CCC ends Some Endonucleases Hae III Pst 1 Smal exon intron exon 1-60 60..648 649.686 ORIGIN 1 catttgtect gatgccagte tagaggatge cagaaaagag gttggteate accttgaact 61 gatacaataa tcagtatgge etttctgtga uttttcaaaa ctgagttett aaagccttta 121 gaaatgtgtt gcatgttatg titactctgt gtcatagtga ctegcagaac tgcacatett 181 gcagtgetgt ggteccttca gtetegteet geticacage aagecaagat cagatttggt 241 tttgcacagt gecagacata gagctttatt ttgtgtgtta gtcageccac caaaacacca 301 tgactggggg caacaaaaaa agettctaat ggaaagetgt agtaggttca gcaaaaacac 361 ttgtettcac aacgcaagag gtaccatttg ettgeggaat ctcttaaatt cactagactt 421 agaaatatgt tetgaaacag actttttagg ctgtteetgg aaatattttt tecataattg 481 gaaataatta tutgetttc aagtgtcagt cagtgaaaga aaagacttet cagcaaagga 541 tegetttget tetcacttca cttagetgta ctggcectaa gacaatgtga cagttacget 601 accctctgtt cagcagaaca actttatctg tttgtttgtt tatttaggg accttacgat 661 gtcattgtce ttectggagg taacct