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Why during the PCR is there a temperature change for certain times and what is the function of these cycles of different
Posted: Tue May 17, 2022 6:35 pm
by answerhappygod
- Why During The Pcr Is There A Temperature Change For Certain Times And What Is The Function Of These Cycles Of Different 1 (65.4 KiB) Viewed 177 times
Why during the PCR is there a temperature change for certain times and what is the function of these cycles of different temperatures? explain. . During the PCR, the temperature change for certain times is designed in order to carry out the exponential amplification of the small DNA fragment by chain polymerization. • Cycles are rendered in a basic three step process which is repeated 25-40 times: • 1st step: separates the DNA double strand for a period of 15-40 seconds at a temperature of 95° C -97° C. • 2nd step: designed primers react with the single stranded DNA thus sticking complementary to other bases in a period of 0.5-2min. at a temperature of 40° C-65° C. • 3rd step: dNTPs are placed between the primers synthesizing complementary sequence to template DNA strands for a period of 1-2 min. at a temperature of 72° C.