Answer Happy

HD Sequencing Series (Chs. 1-7) A. GAPDH and DNA Isolation Techniques 1. The GAPC gene family includes genes coding for various GAPDH isozymes. Why clone the GAPDH gene? 2a. Where is genomic DNA found in eukaryotic cells? b. What other types of intracellular compartments contain DNA in eukaryotes? 3. During this project we isolated/extracted genomic DNA from onion tissue and plasmid DNA from bacterial cells. Compare and Contrast the methods used for each type of isolation. [Consider the following: How did the source of DNA (for example, plant tissue vs E.coli cells) effect the isolation technique? How did the size of the DNA molecules being isolated factor in?] 4. What type of reagent is generally used to break down cell membranes? 5. What chemical reagent is generally used to precipitate DNA? B. Polymerase Chain Reaction