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The following tables contain the results from the experiment as described in the Materials and Methods. The task in this Results section is to perform the necessary calculations for cell density (CFU/mL) of three organisms after incubation at different temperatures, use the enumeration data to draw conclusions regarding optimal growth temperatures. 3. Calculate the final dilution factors for tubes 3 and 4 in Table 1 below. (2 pts., 1 point each) Table 1. Serial Dilution. The individual and final dilution factors for each tube in the serial dilution used in this study. Dilution Tube # Individual Dilution 10'10' 10 10 10 10 Factors Final Dilution Factors 10'10 Answer 10* Answer 10 Table 2. Colony counts after spread plating 0.1 mL from each dilution tube in Table 1 onto its corresponding TSA plate. Plate # 1 2 3 4 5 6 Organism 1,4°C TNTC TNTC TNTC TNTC 171 21 Organism 2, 35°C TNTC TNTC TNTC TNTC 237 19 Organism 3, 55°C TNTC TNTC 241 11 1 0 Calculations and Conclusions Questions 4. Complete Table 3. Transfer the data for Organisms 1, 2, and 3 from Tables 1 and 2. (6 pts., 1 point each) Table 3. Data needed to calculate the cell concentrations (CFU/mL) for Organisms 1, 2, and 3 grown at 55°C, 35°C and 4°C to complete Table 4. Final Dilution Factor Plate of Tube Organism 1(4°C) Answer 0.1 mL Answer Organism 2 (35°C) Organism 3 (55°C) # Colonies on Usable Volume Plated Answer 0.1 ml Answer Answer 0.1 ml Answer

5. Complete Table 4. Use the information from Table 3 to calculate the missing CFU/mL data for Organisms 1, 2, and 3. Show the calculations in the space provided on the next page for partial credit. (6 points, 2 points each) Table 4. Calculated cell densities (CFU/mL) for each of the tested organisms incubated overnight at 4°C, 35°C, and 55°C. Each organism was grown and assayed in triplicate. Incubation Temperature Organism 1 Organism 2 Organism 3 2.4 x 10 3.7 x 10 1.13 x 10 1.64 x 10 2.4 x 10 1.72 x 104 Calculated Answer 2.12 x 10 1.43 x 10 4°C 4°C 4°C 35°C 0 4.6 x 10 5.7 x 10% 4.3 x 10 35°C 0 6.5 x 10 35°C 0 Calculated Answer 55°C 0 0 5.2 x 10 1.61 x 10 2.01 x 10 55°C 0 0 55°C 0 0 Calculated Answer Example Calculations for Table 4 (For partial credit, feedback) • Organism 1 Calculation • Organism 2 Calculation • Organism 3 Calculation