Q10. Imagine a phage titration was performed using a different dilution scheme. • Two ml of the water sample were added
Posted: Tue May 17, 2022 3:26 pm
Q10. Imagine a phage titration was performed using a different dilution scheme. • Two ml of the water sample were added to 8 ml of sterile diluent, producing dilution A. • Two ml of dilution A were added to 18 ml of sterile diluent, producing dilution B. • Five ml of dilution B were added to 5 ml of sterile diluent, producing dilution C. Then 0.1 ml of each dilution were mixed with 0.5 ml of E. coli strain B culture and soft agar. The mixture was poured onto nutrient agar plates, labeled A, B and C (each corresponding to a dilution). Two days later, the following phage counts were recorded: Plaque Plate counts ΙΑ 285 B 30 С 14 Based on these results, what is the phage titer in the water sample?