Answer Happy

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QUESTION 31 Which of the following statements is/are correct regarding regulation of the eukaryotic cell cycle? Checkpoints delay the cell cycle in order to repair damage to the genome Most steps of the cell cycle require successful completion of the previous step O Cyclin-CDK complexes regulate the cell cycle in all eukaryoles O All of the above QUESTION 32 Mice usually have wild-type agouti fur that appears grey, but a mouse geneticist has a pure-breeding white-furred strain that is homozygous for a recessive mutation. Molecular analysis shows that the mutation is a missense mutation in an exon common to three alternatively spliced forms of a gene expressed in hair follicles. Which of the following transgenic animals would be most useful to determine which spliced form (or forms) is sufficient to specify agouti fur? O Express a wild-type version of a cDNA for each spliced form in the white mutant mouse to see which form(s) rescue the phenotype. Introduce the wild-type genomic allele into the mutant mouse to test for rescue of the mutant phenotype. O Use CRISPR/Cas9 to make a large deletion of the entire coding region of the identified gene. O Construct a GFP reporter using the promoter for the identified gene QUESTION 33 One concern about using genetically-modified organisms that many of the methods used to create them introduce into the genome DNA from a different species (l.e. foreign DNA). Which of the following methods has the lowest potential of introducing foreign DNA into the genome? O Microinjection of a transgene into a mouse pronucleus O Deletion mutations introduced by CRISPR/Cas9 O A gene knockout in mouse using homologous recombination in ES cells