Answer Happy

​​​​​​​Please answer all questions PLEASE. Thank you.
QUESTION 26 Below is the sequence of mouse DNA that contains a microsatellite sequence (only one strand is shown that reads 5 to 3"). You wish to PCR amplify DNA from this locus to see if the length of the microsatellite varies 5'-CACTCTCGCTTTAAGCGAGCTAGATAATGCTCTTTTGCTTTCTCCAGTCTCAGC CTCCCTCCTTTCCAGACACATCCATTATACTGCCAAAATCGAATTAGGGTTAAGAAT between different mouse strains. AACAAGAGTAAGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG 3 AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGATCGAATTCACTCACTGTAGAGA 4 AGAGCTTGGTGAGTGAATTGGTGGAAGATGGAGAGAAATCGATGTAGGCAAAGG—3 Of the four locations of PCR primer pairs indicated which would be the best to use? (5—>3' indicates that the primer sequence would be as written above; 3'<–5' indicates that the primer corresponds to the complement of the sequence shown.) O 1 (5'->3') and 4 (5'->3') 0 1 (5->3") and 4 (3'45') O 1(3-5') and 4 (5-3) O 2 (5'->3") and 3 (5'->3) O 2(5—>3") and 3 (3'<--5') QUESTION 27 Which type of DNA construct could be used in a transgenic mouse strategy to study a selected mouse gene's expression pattern in a whole animal? O A reporter gene can be expressed using a synthetic (not natural) promoter so that it can be expressed in all tissues of an organism. The selected gene can be expressed under the control of synthetic (not natural) promoter so that it is expressed in all tissues of the mouse. O A gene not normally found in the mouse genome can be expressed under the control of synthetic (not natural) promoter so that it is expressed in all tissues of the mouse. O A reporter gene can be cloned so that it is under the control of the selected gene's cis-regulatory region.