Answer Happy

You are studying a (hypothetical) novel gene, which is related
to the development of the nervous system,
in Drosophila. Propose an experimental design
that allows you to carry out basic studies of gene expression and
function, as well as the next steps in the analysis of the gene of
interest.
Make sure your gene expression studies include mRNA level and
protein level expression studies. Also, your genetic function
studies should include loss-of-function studies as well as
gain-of-function studies. For these studies, mention the techniques
to be used, including the key substances you need to obtain, so
that your analyzes are specifically directed to your gene of
interest.
A little help: Some of the techniques to use should be
CRISPR-Cas9 and in situ hybridization. It must also be demonstrated
how it will be determined that the expression is in
the nervous system.