Answer Happy

PART I (Lab data required). 4) points 1) Write down the results from your transformations by filling this table (same as p.81): Tube# dilution Transform- ants/ml # mis type of plate: Number of plated Colonies on the plate 0.1 No Antibiotic 1000 (white) Average total (transform transform- ants/ml) ants xxxxxxxx xxxxxxxx XXXXXXX 0.1 T1 None (no DNA) T1 None (no DNA 10(-1) T2 (Native) 10(-2) Amp + IPTG No colonies +X-gal XXXXXXXX XXXXXXXX XXXXXXX 0.1 0.1 None 0.1 T3 (RC) None 0.2 None 0.1 T4 (LC) None 0.2 Amp + IPTG 612 (blue) +X-gal Amp + IPTG 87 (blue) +X-gal Amp + IPTG 1 (blue) +X-gal Amp + IPTG 3 (blue) +X-gal Amp + IPTG 580(blue) +X-qal Amp + IPTG 908 (blue) +X-gal Amp + IPTG 109(1 blue + +X-gal 108 white) Amp + IPTG 157(1 blue + +X-gal 156 white) Amp + IPTG 164 (155blue +X-gal +9white) Amp + IPTG 466(450 blue+ +X-gal 16 white) Amp + IPTG 589(582 blue + 7 white) Amp + IPTG 606 590 blue +X-qal + 16 white) None 0.1 T5 (PC) None 0.2 None 0.05 None 0.1 T6 (L) None 02 +X-gal None 0.2

1) Write down the results from your transformations by filling this table (same as p.81): T2 Tube # dilution # ls type of plate. Number of Transform Average total plated Colonies on ants/ml (transform transform- the plate ants/ml) ants T1 None 0.1 No Antibiotic 1000 (white) (no XXXXXXXX XXXxxxxx xxxxxxx DNA T1 None 0.1 Amp + IPTG No colonies (no +X-gal XXXXXXXXXXXXXXXX XXXXXXX DNA) 10(-1) 0.1 Amp + IPTG 612 (blue) +X-gal (Native) 10(-2) 0.1 Amp + IPTG 87 (blue) +X-gal None 0.1 Amp + IPTG 1 (blue) T3 X-gal (RC) None 0.2 Amp + IPTG 3 (blue) #X-gal None 0.1 Amp + IPTG 580(blue) X-gal (LC) None 0.2 Amp + IPTG 908 (blue) *X.gal None 10.1 Amp + IPTG 109(1 blue + X-gall 108 white (PC None 0.2 Amp + IPTG 1571 blue + +X-gal 156 white) None 0.05 Amp + IPTG 164 (155blue +X-gal +9white None 0.1 Amp IPTG 466(450 blue T6 .X gal 16 white (L) None 0.2 Amp + IPTG 589(582 blue X gal +7 white None 0.2 Amp IPTG 606 590 blue X-qal 16 white TA T5