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Question 11a. When a PCR reaction is performed using genomic DNA as a template, a 1.5 kb product is amplified. When the
Posted: Tue May 17, 2022 1:41 pm
by answerhappygod
- Question 11a When A Pcr Reaction Is Performed Using Genomic Dna As A Template A 1 5 Kb Product Is Amplified When The 1 (25.64 KiB) Viewed 114 times
Question 11a. When a PCR reaction is performed using genomic DNA as a template, a 1.5 kb product is amplified. When the same reaction is set up using cDNA as a template, a 0.8 kb product is amplified. The most likely explanation for the different sized products is: There was an exon in the genomic DNA that was spliced out of the mRNA before it was converted to cDNA Contamination of the genomic DNA Degradation of the mRNA before it was converted to CONA. There was an intron in the genomic DNA that was spliced out of the mRNA before it was converted to cDNA The individual is heterozygous