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Question 11a. When a PCR reaction is performed using genomic DNA as a template, a 1.5 kb product is amplified. When the
Posted: Tue May 17, 2022 1:37 pm
by answerhappygod
- Question 11a When A Pcr Reaction Is Performed Using Genomic Dna As A Template A 1 5 Kb Product Is Amplified When The 1 (46.07 KiB) Viewed 154 times
Question 11a. When a PCR reaction is performed using genomic DNA as a template, a 1.5 kb product is amplified. When the same reaction is set up using cDNA as a template, a 0.8 kb product is amplified. The most likely explanation for the different sized products is: Contamination of the genomic DNA. The individual is heterozygous. There was an intron in the genomic DNA that was spliced out of the mRNA before it was converted to cDNA. Degradation of the mRNA before it was converted to cDNA. There was an exon in the genomic DNA that was spliced out of the mRNA before it was converted to cDNA.