Answer Happy

Question 11a. When a PCR reaction is performed using genomic DNA as a template, a 1.5 kb product is amplified. When the same reaction is set up using cDNA as a template, a 0.8 kb product is amplified. The most likely explanation for the different sized products is: Contamination of the genomic DNA The individual is heterozygous, There was an exon in the genomic DNA that was spliced out of the mRNA before it was converted to cDNA. There was an intron in the genomic DNA that was spliced out of the mRNA before it was converted to CONA Degradation of the mRNA before it was converted to cDNA,

Question 11b. A PCR reaction amplifies two DNA fragments from a genomic DNA template. All of the following could explain this observation EXCEPT: The annealing temperature was too low decreasing specificity. The individual was heterozygous for two different sized alleles. The reaction was contaminated with a foreign source of DNA The denaturation temperature was too low decreasing specificity The primers were not specific and amplified two different sequences