Please can someone help me with thee questions, i have been struggling for 3 days with them and really need assistance,
Posted: Tue May 17, 2022 1:16 pm
Please can someone help me with thee questions, i have been
struggling for 3 days with them and really need assistance, i would
appreciate it if someone answered before tomorrow. Thank you,
please help!!!!!!
1) The complementarity determining loops of an antibody are
responsible for the identification of the antigen using specific
epitope. Explain how the loops facilitate the recognition of
epitopes.
2) Briefly explain how the protein cleavage method for a
globular protein would differ from a membrane protein in
preparation for Liquid Chromatography-Mass Spectrometry (LC-MS)
experiments.
3) What general property would be expected of a liquid
chromatograhy (LC) column on which protein samples are being
separated for analysis by mass spectrometry? Briefly explain why
the column would have that property.
4) Briefly explain the importance of a positive control when
using chemical cleavage methods
5) Compare 2D polyacrylamide gel electrophoresis to LC-MS as a
proteomics technique to identify differences between patients with
diseases and controls without diseases.
struggling for 3 days with them and really need assistance, i would
appreciate it if someone answered before tomorrow. Thank you,
please help!!!!!!
1) The complementarity determining loops of an antibody are
responsible for the identification of the antigen using specific
epitope. Explain how the loops facilitate the recognition of
epitopes.
2) Briefly explain how the protein cleavage method for a
globular protein would differ from a membrane protein in
preparation for Liquid Chromatography-Mass Spectrometry (LC-MS)
experiments.
3) What general property would be expected of a liquid
chromatograhy (LC) column on which protein samples are being
separated for analysis by mass spectrometry? Briefly explain why
the column would have that property.
4) Briefly explain the importance of a positive control when
using chemical cleavage methods
5) Compare 2D polyacrylamide gel electrophoresis to LC-MS as a
proteomics technique to identify differences between patients with
diseases and controls without diseases.