A discussion is needed on this SDS-PAGE, any help?
Posted: Tue May 17, 2022 1:09 pm
A discussion is needed on this SDS-PAGE, any help?
Lane 1 MWM Lane 2 CTL Lane 3 Lane 4 Lane 5 unknown unknown unknown mowe Lane 6 unknown Lane 7 Lane unknown unknown Mm MW Da 5 210000 10 125000 15 101000 35 56200 60 35800 $ 29000 120 21000 140 18000 170 000 220
Protein (MW) (Log MW) Migrated Retention factor distance of MWM (mm) 5 5/220-0.02278 10 10/220=0.0455 Marker 1 210000 Marker 2 125000 Marker 3 101000 15 15/220-0.0682 Marker 4 56200 35 Marker 5 35800 60 Log(210000)-5.3222 Log(125000)=5.0969 Log(101000)-5.0043 Log(56200)-4.7497 Log(35800)=4.5539 Log(29000)=4.4624 Log(21000)= 4.3222 Log(18000)= 4.2553 Log(6900)= 3.8388 Table 1 35/220=0.1591 60/220=0.2727 85/220-0.3864 Marker 6 29000 85 120 Marker 7 Marker 8 21000 18000 120/220=0.5455 140/220-0.6363 140 Marker 9 6900 170 170/220=0.7727 My standard Curve 5.5 y = -1.6263x + 5.1485 R= 0.9322 15 Log 10 Value 35 3 0 0.2 0.8 1 생 04 0.6 RF Value Graph 1
Results Helow shows the electrophoresis gel stains. Lane 1 contains the molecular weighe market and Jane 2 contains the mix control. Lane 3 is FT2 HIC Lane 4 is E2 HIC while lane 5 E3 HIC Lane 6s CC2 IEX, 7 is CCJIEX and finally lane is 192 IEX La MWM La CTL LLL w ken La www Mm MW Du 21 10 12000 1101000 13 56700 300 11 000 120 2100 100 000 170
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Protein (MW) (Log MW) Migrated Retention factor distance of MWM (mm) 5 5/220-0.02278 10 10/220=0.0455 Marker 1 210000 Marker 2 125000 Marker 3 101000 15 15/220-0.0682 Marker 4 56200 35 Marker 5 35800 60 Log(210000)-5.3222 Log(125000)=5.0969 Log(101000)-5.0043 Log(56200)-4.7497 Log(35800)=4.5539 Log(29000)=4.4624 Log(21000)= 4.3222 Log(18000)= 4.2553 Log(6900)= 3.8388 Table 1 35/220=0.1591 60/220=0.2727 85/220-0.3864 Marker 6 29000 85 120 Marker 7 Marker 8 21000 18000 120/220=0.5455 140/220-0.6363 140 Marker 9 6900 170 170/220=0.7727 My standard Curve 5.5 y = -1.6263x + 5.1485 R= 0.9322 15 Log 10 Value 35 3 0 0.2 0.8 1 생 04 0.6 RF Value Graph 1
Results Helow shows the electrophoresis gel stains. Lane 1 contains the molecular weighe market and Jane 2 contains the mix control. Lane 3 is FT2 HIC Lane 4 is E2 HIC while lane 5 E3 HIC Lane 6s CC2 IEX, 7 is CCJIEX and finally lane is 192 IEX La MWM La CTL LLL w ken La www Mm MW Du 21 10 12000 1101000 13 56700 300 11 000 120 2100 100 000 170