You obtained the following raw data when setting up a Bluet standard curve: BSA (mg/ml) OWN-O 2 3 4 5 6 Absorbancy 540nm
Posted: Tue May 17, 2022 12:50 pm
You obtained the following raw data when setting up a Bluet standard curve: BSA (mg/ml) OWN-O 2 3 4 5 6 Absorbancy 540nm 0.158 0.210 0.260 0.305 0.360 0.410 0.455 0.510 0.530 0.550 0.554 7 8 9 10 After blanking against a biuret-dH20 sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.249 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg/ml (up to 3 significant figures).