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QUESTION 6 (10 total marks] The Michaelis-Menten reaction mechanism between enzyme (E) and substrate (5) is: E+ SES P+E
Posted: Tue May 17, 2022 10:14 am
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- Question 6 10 Total Marks The Michaelis Menten Reaction Mechanism Between Enzyme E And Substrate 5 Is E Ses P E 1 (57.5 KiB) Viewed 57 times
QUESTION 6 (10 total marks] The Michaelis-Menten reaction mechanism between enzyme (E) and substrate (5) is: E+ SES P+E The Michaelis-Menten equation for the rate of product formation is imax r= 1+ KM/[S]. where [S]. is the initial concentration of the substrate and Km is the Michaelis constant as defined below: katko KM ka (a) Define the Michaelis constant in terms of [S]. by deriving an appropriate relation. (3 marks) (b) Define the catalytic efficiency of an enzyme, n, and briefly explain how it varies with the reaction rate constants. Specify the conditions under which the maximum value of n can be achieved. (3 marks) (c) If the rate of reaction is 0.41 mmol dm's1 when the substrate concentration is 0.20 mmol dm?, what is the maximum rate max of this reaction? The Michaelis constant is KM = 0.75 mmol dm? (4 marks)