Answer Happy

4 Metformin is a first line treatment for type 2 diabetes. To detect the drug using liquid-chromatography with mass spectrometry:- Would you use positive or negative ion mode and give reason [2 marks]. b. Would you choose an acidic, basic or neutral mobile-phase and give reason. [2 marks]. a NH NHI 'N N1 H NH2 Metformin (FM 129.167) C. d. Metformin elutes close to an impurity with a mass of 129.300. Calculate the resolving power of mass spectrometer required to detect both ions. [4 Marks] Pharmacokinetic profile of the drug was determined in two patients. Plasma samples were taken from patients from point of administration and thereafter for 12 h. The samples were subsequently analysed using tandem mass spectrometry. Standards of the drug in the concentration range 3.125 to 100 ug/mL were also analysed. Use the data provided to determine the concentration of drug in patient samples and use this to plot graphs showing how the concentration of drug in plasma varies with time. [12 Marks] Concentration of metformin (vg/mL) 3.125 6.25 12.5 25 31.25 50 HPLC-=Peak area (arbitary units) 76710 153400 307685 614786 791020 1228990 100 2456780