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2. Answer the following questions using the figure below for the denaturation(i.e., melting) of the protein Lysozyme. a.
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- 2 Answer The Following Questions Using The Figure Below For The Denaturation I E Melting Of The Protein Lysozyme A 1 (51.75 KiB) Viewed 52 times
2. Answer the following questions using the figure below for the denaturation(i.e., melting) of the protein Lysozyme. a. What is the melting temperature (Tm) at pH 2.0, 2.5, and 4.0? b. Is the native protein more stable at pH 2.0 or 4.0? Lysozyme Unfolding 1.9 ACO 1.5 specific heat capacity Ср cal/gK pH 4 pH 2.5 pH 2.0 Codenatured 1.1 Cp native DA A 0.7 дер 0.3 1 1 20 30 1 40 1 50 1 70 60 90 80 Tm T(°C) Privalov and Khechinashvilli. J. Mol. Biol. 86:665 (1974)