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View Review New Tab New Tau erences Mailings New lad Developer Help Figure 1 - A chromatograph of a two-component mixture, X and Y. 18 16 Х 14 14 12 10 Detector response 8 ^ 6 4 2 0 60 65 70 50 55 0 40 45 5 15 20 25 10 30 35 Time (minutes) A chromatograph of a two-component mixture is shown above in Figure 1. The LC column is 30-cm long. The flow rate was 0.30 ml/min. Using the chromatograph determine/calculate the following: Q4) - The time components X and Y spends in the stationary phase (4 marks). Q5) - The area under the curve (AUC) for components X and Y (6 marks). Q6) - The retention factor for components X and Y (4 marks). Q7) - A GC separation was conducted on an analytical sample containing a pesticide organophosphate, Malathion. This sample was treated with an internal standard of Chlorobenzene, giving a concentration of 15.0 ppm. A 1.0 ML injection onto the GC gave a Flame ionization detector response of 1012 for Chlorobenzene, and 3529 for Malathion. A 1.0 PL standard solution of 30.0 ppm Chlorobenzene with 15.0 ppm of Malathion was injected giving a response of 758 and 2644 respectively. What is the concentration of Malathion in the Sample? (12 marks).