QUESTIONS: (1) If your mobile phase flow-rate was set to 0.5 mL/min and it took 2 minutes for the non-bound material to
Posted: Mon Nov 15, 2021 4:43 pm
QUESTIONS: (1) If your mobile phase flow-rate was set to 0.5 mL/min and it took 2 minutes for the non-bound material to exit the column, what would be the volume of the column. (2) HPLC is a non-destructive technique. What advantage is this compared to GC separations? (3) What are some advantages to an internal standard method for the analysis of samples? (4) What is an advantage in using a diode array detector compared to a fixed wavelength detector?